Bioline cdna synthesis kit
WebJan 31, 2013 · cDNA synthesis was performed with Tetro Reverse Trancriptase kit (Bioline Ltd.) according to the manufacturer’s recommendation. Quantitative PCR was performed using real-time PCR (ABI PRISM 7900, Applied Biosystems). ... reverse transcription reaction using the designed miRNA specific stem loop-RT primer and … Product Highlights. Efficient – high-target affinity, coupled with a novel TransAmp buffer system for improved yield of full-length cDNA; Unbiased – optimized mix of random he See more
Bioline cdna synthesis kit
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WebContent And Storage. Contents: The kit includes reagents and buffers for 10 first- and second-strand cDNA synthesis reactions converting 25-50 µg total RNA or 0.2-5 µg mRNA into double-stranded cDNA. Storage: Store … WebSensiFAST™ cDNA Synthesis Kit has been developed to generate high-quality first strand cDNA from total RNA template. SensiFAST™ cDNA Synthesis Kit provides a rapid and …
WebContent And Storage. Contents: The kit includes reagents and buffers for 10 first- and second-strand cDNA synthesis reactions converting 25-50 … WebThe SensiFAST cDNA Synthesis Kit is delivered to your lab under blue ice or on top of dry ice. Please note that an additional fee may apply for dry ice packaging and handling. ... Supplier: Bioline reagents. Catalog number: BIO-65054. Size: 250 Reactions. Price: 0 USD Send. Add product to cart. 01013807754. SensiFAST cDNA Synthesis Kit.
WebJan 4, 2024 · SensiMix SYBR & Fluorescein Kit (QT615‐20) was purchased from Bioline. ... cDNA was synthesized using the iScript cDNA Synthesis Kit following manufacturer's protocol. Briefly, an iScript master‐mix was prepared by mixing four volumes of 5× iScript Reaction Mix with one volume of iScript Reverse Transcriptase. Subsequently, 5 µL of ... WebQuantiNova Reverse Transcription Kit. For fast cDNA synthesis and reproducible real-time two-step RT-PCR. QuantiNova RT-PCR Kits. For highly sensitive and specific one-step qRT-PCR and multiplex qRT-PCR …
WebThe synthesis of cDNA was performed as per instructions from the SensiFASTTM cDNA Synthesis kit (Bioline). The cDNA and targeted gene primers were mixed with dNTPs, buffer, and SYBR Green Master Mix (Thermo Fisher Scientific, AU) in a 386-well plate at 10 μL per well as protocol indicated. Forty cycles of real-time qPCR were detected by a ...
WebApr 13, 2024 · Lipofectamine 2000, Tetro cDNA synthesis kit and QuickExtract DNA extraction were ordered from Invitrogen (USA), Bioline (Germany), and Lucigen (USA), respectively. Primary and secondary antibodies, and fluorescence-tagged secondary antibodies were purchased from Santa Cruz Biotechnology (USA). Midorigreen was … jeff\u0027s garage door repairWebFor RT-PCR, RNA-seq, and other applications, LunaScript RT Master Mix Kit (Primer-free) ( NEB #E3025) is an optimized master mix containing all the necessary components for first strand cDNA synthesis, except for primers. The mix is compatible with random primers, oligo dT primers, and gene-specific primers, enabling maximum cDNA synthesis ... jeff\u0027s gardenWebThe SensiFAST SYBR® No-ROX Kit has been optimized to deliver optimal performance in tandem with the SensiFAST cDNA Synthesis Kit, which offers fast, unbiased cDNA synthesis, without compromising cDNA yield or coverage. Applications. Gene expression analysis; DNA / RNA target detection; miRNA profiling / quantification; Copy number … jeff\u0027s garageWebProduct information: miRNA cDNA Synthesis Kit with Poly A Polymerase Tailing - Gentaur.com - Product info. miRNA cDNA Synthesis Kit with Poly(A) Polymerase Tailing. Catalog number. G902. Supplier. ... Supplier: Bioline reagents. Size: 250 Reactions. Price: 916.5 USD. Tetro cDNA Synthesis Kit. Catalog number: BIO-65042. Supplier: Bioline ... jeff\u0027s garage potomac ilWebExcellent results from a variety of samples. Genomic DNA was isolated in duplicate from E. coli, mouse tail, mouse lung, HeLa cells and 3T3 cells, using ISOLATE II Genomic DNA Kit (lanes 1-5 and 6-10 respectively) … jeff\\u0027s freezette menuWebSensiFAST SYBR ® Lo-ROX Kit is designed for fast, highly sensitive and reproducible real-time PCR and has been validated on commonly used real-time instruments. The antibody-mediated hot-start DNA polymerase system reduces the chances of primer/dimers formation. This allows a much greater dynamic range by removing competition for … lagu takbiran idul fitriWebThe SensiFAST™ cDNA Synthesis Kit provides a rapid and sensitive method for first-strand cDNA synthesis, which displays excellent linearity across a wide range of starting material. This gives the same relative representation in cDNA templates, regardless of gene abundance, making it excellent for use in qPCR studies. lagu takbiran h muammar za mp3