Blend taq toyobo
Web19 PCR Buffer (Toyobo, Osaka, Japan), 0.2 mM of dNTPs, 0.2 lM of each primer and 1.25 U Blend Taq (Toyobo). DNA amplification was performed in a MyCy-cler Thermal Cycler (Bio-Rad, Hercules, USA). The PCR profile consisted of denaturation at 96 C for 5 min, fol-lowed by 36 cycles of 94 C for 45 s, 50 C for 30 s, and 72 C for 90 s. WebPCR amplification reactions with primers A09, A11, A13, A17, A18 were carried out in a total reaction volume of 10 μL containing 1 × Taq buffer, 10 ng genomic DNA, 0.25 mM dNTPs, 5 pmol primers, and 0.5 units Blend Taq-Plus DNA polymerase (Toyobo, Osaka, Japan).
Blend taq toyobo
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WebCopy caption. Embed figure. Detection of mutations in RsGL1a and RsGL1b of T 0 generation plants. WebToyobo has various NGS related products as shown below. The detailed information can be obtained by going to the linked sites. Applications Product Name; Library preparation: GenNext™ NGS Library Prep Kit: ... Blend Taq™ & Blend Taq™ -Plus; anti-Taq high; dNTPs Mixture (2 mM)
WebSep 11, 2012 · The reaction mixtures for the nested PCR analysis contained 5 μl of 10× PCR Buffer for Blend Taq (TOYOBO), 5 μl of dNTPs (2 mM each), 2 μl of nifH3, 2 μl of nifH4, 0.5 μl of Blend Taq (2.5 U μl −1; TOYOBO), and 34.5 μl of sterile Milli-Q water. The first round of nested PCR consisted of an initial 5-min denaturation at 95 °C ... WebNov 4, 2008 · The reaction solution (50 μl) contained 1 μl cDNA, 1 × PCR buffer, 0.2 mM each dNTP, 0.5 μM of each primer, and 2.5 units Blend Taq (TOYOBO, Japan). The amplification conditions were as follows: 5 min at 95 °C, followed by 30 or 35 cycles of 30 s at 95 °C, 60 s at 40–54 °C, 60 s at 72 °C, and 5 min at 72 °C for each gene as shown in ...
WebMar 7, 2024 · The PCR reaction mixture (20 µl) contained 2 µl of 10× Blend Taq Buffer (Toyobo Osaka), 1.6 µl of dNTPs, 0.5 µl of each primer, 1 µl of genomic DNA template, 0.2 µl of Blend Taq, and 14.2 µl of sterile distilled water. The PCR conditions and primers used are shown in Table 1. Genomic sequencing and comparative genomic analysis Webdone using the DNA polymerase of Blend Taq (Toyobo, Tokyo, Japan) under standard conditions as described in refs. 18 and 19. The amplified genomic DNA fragment was sequenced to be 804bp. As expected, the 804-bp ... (Toyobo) were used. The majority of the cDNA sequences were obtained by usual PCR with primers 7 and 8 (Fig. 1) and …
WebBlend Taq® / Blend Taq® -Plus- - Toyobo. EN. English Deutsch Français Español Português Italiano Român Nederlands Latina Dansk Svenska Norsk Magyar Bahasa …
WebBLEND BRIE SANDWICH. Brie, green apple, turkey, pesto and honey made on a lightly toasted ciabatta roll. EGG SANDWICH. Our signature breakfast sandwich. Made with … bridal brunch table settingshttp://www.bio-toyobo.cn/pdf/BTQ-1_2e.pdf canterbury st hughesdaleWebAug 15, 2006 · This exclusive blend cycle technology creates amazing blends with the touch of a button. Care to crush ice? The Total Blender Classic has a 1560 watt motor … bridal brunch shower foodWebDec 17, 2024 · Event starts on Saturday, 17 December 2024 and happening at Blend Coffee Bar, Ashburn, VA. Register or Buy Tickets, Price information. German Christmas … bridal brunch thrown by brideWebUnit Definition : One unit of enzyme is defined as the amount of enzyme that will incorporate : 10 nmoles of dNTPs into acid insoluble material in 30 minutes at 75 canterbury street stables playgroupWebThermo T7 RNA Polymerase Buffer. Thermo T7 RNA Polymerase Buffer. More Info. Showing 1-20 of 24 item (s) 1. 2. Can't find the products you are looking for? Contact us at [email protected]. canterbury street motWebThis kit includes the following components for 250 reactions, 20 μL total reaction volume. All reagents should be stored at -20°C. < HSTTX-101 >. 2x Buffer for rTth/ TTx (DNA) 1.25 mL x 2. Hot Start TTx DNA Polymerase (4U/ μL) 62.5 μL. Note: 2x Reaction Buffer contains essential components for the reaction (buffer, salts, Mg2+, dATP, dCTP ... bridal buddy wedding gown underskirt