Fgh1tutg
WebOct 2, 2024 · To produce lentiviruses, FgH1tSHP2 or FUCas9Cherry plasmids were transiently cotransfected into HEK293T cells with the packaging plasmids pRSV-Rev, pMDLg/pRRE, and pCMV-VSV-G (all from Addgene) using FuGENE HD Transfection Reagent (Promega Corporation). WebJan 11, 2024 · FgH1tUTG (Addgene plasmid # 70183) using BsMB1 enzyme sites. Lentivirus was produced using the same protocol as described for shRNA knockdown and infected into Jurkat cells that stably expresses Cas9. After 3 days, cells were analyzed with a BD™ LSR II flow cytometer. Dual-positive eGFP and mCherry cells were sorted using …
Fgh1tutg
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WebShimobi Onuoha. Simon Thomas. Daniela Soriano Pignataro. Persis J Amrolia. View full-text. ... G, pRSV-Rev, pMDLg/pRRE, and the E7 gRNA containing FgH1tUTG plasmid using the Lipofectamine 3000 ... WebThis website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.
WebFgH1tUTG Citations FgH1tUTG Citations (30) Plasmid Article: An inducible lentiviral guide RNA platform enables the identification of tumor-essential genes and tumor-promoting mutations in vivo. WebJul 4, 2024 · Abstract. The protein SAMSN1 was recently identified as a putative tumor suppressor in multiple myeloma, with re-expression of Samsn1 in the 5TGM1/KaLwRij …
WebThe doxycycline (dox)-inducible gRNA expression plasmid vector, FgH1tUTG (Addgene #70183), was kindly provided by Professor Marco Herold (Walter and Eliza Hall Institute of Medical Research ... WebSep 16, 2024 · NMuMG cells were first infected using 8 μg/ml polybrene with lentiviral particles containing the FUCas9Cherry vector. Single Cherry-positive cells were then sorted and grown in 96-well plates. One clone was chosen to be subsequently infected with lentiviral particles containing FgH1tUTG-sgHmg20a or FgH1tUTG-sgPhf14.
WebPlasmid FgH1tUTG_hup53_Ex5 from Dr. Marco Herold's lab contains the insert hup53 Ex5 and is published in Unpublished This plasmid is available through Addgene. Image: …
WebFgH1tUTG_mup53_Ex5. Plasmid #85535. Purpose. Inducible expression of guide RNA (mup53_Ex5) with fluorescent GFP reporter. Depositor. Marco Herold. Article. Aubrey et … clovers overseas studiesWebLentiviruses containing sgRNAs in the doxycyclineinducible FgH1tUTG backbone (Addgene Plasmid #70183) were produced and cells were transduced with these vectors using established methods [15 ... clovers outfitWebPlasmid sequence and annotations. Use text editor or plasmid mapping software to view sequence. SnapGene File: Plasmid sequence and SnapGene enhanced annotations. … clover south carolina weatherWebFgH1tUTG Sequences (4) Addgene Sequences: Full (1) Partial (3) Full Sequences from Addgene (1) Based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from … clover soy milkWebApr 11, 2024 · FgH1tUTG-sgRNA viruses were further transduced into these K562-Cas9/dCas9 stable lines and sorted for GFP positives. To generate EZH2 KO, in vitro synthesized sgRNA (Synthego) was transiently ... cabbage rolls recipe porkWebFgH1tUTG_huBim_Ex3 Sequences (1) Addgene Sequences: Full (1) Full Sequences from Addgene (1) Based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences and/or Sanger results (Addgene Assembled Sequence). Analyze Sequence GenBank SnapGene File Help clover sowing rateWebNov 25, 2024 · To control for effects of doxycycline treatment, potential spontaneous expression of the sgRNA vector (FgH1tUTG can be “leaky” in some cell types) and … cabbage rolls with bread crumbs