Hoechst biology
NettetFigure 1 XL765 suppressed GBM cell viability by inducing apoptosis. (A) The CCK8 assay analysis for the survival of A172, U87, and T98G cells treated with different doses of XL765 for 24 and 48 hrs.(B) The 24and 48 hrs IC50 values of XL765 in A172, U87, and T98G cells.(C) The cell viability of A172, U87, and T98G cells treated with different … NettetHoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish condensed pycnotic nuclei in apoptotic cells and for cell cycle studies in combination with BrdU. It is also available in solution (H-3570).
Hoechst biology
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NettetHoechst 33342 is a well-characterized blue-emitting fluorescent compound widely utilized for nuclear staining. Hoechst 33342 permeates cell membranes and binds to the minor groove of A/T-rich dsDNA sequences, thus preferentially staining nuclei (Figure 1). Fig.1. Hoechst 33342 (in blue) binding dsDNA. Hoechst 33342 Excitation / Emission Spectra NettetHoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish condensed pycnotic nuclei in apoptotic cells and for cell-cycle studies in combination with BrdU. Also available as a room-temperature-stable, ready-to-use solution: NucBlue ...
NettetHoechst 33342 is a bis-benzimide derivative that binds to AT-rich sequences in the minor grove of double-stranded DNA. The emission is very bright, and for the purposes of the … Nettet10. jan. 2024 · Hoechst stains are also cell permeable and can therefore be used in fixed and living cells. They exhibit lower toxicity than DAPI. A membrane-impermeable DNA stain is Propidium-Iodide which is often used to differentiate between living and dead cells in a cell culture because it cannot enter an intact cell.
NettetReferences for Hoechst 33342. References are publications that support the biological activity of the product. Portugal and Waring Assignment of DNA binding sites for 4',6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). NettetFeatures of Hoechst 33342 Fluorescent Stain: • Hoechst dye —blue fluorescent stain specific for DNA (i.e., nuclei of eukaryotic cells) • Convenient —provided as an easy-to-use Hoechst dye solution (20 mM) • Cellular imaging —cell-permeable dye is effective for fixed-cell and live-cell staining. • Counterstain —ideal for use ...
NettetHoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish …
NettetThe following representative example, Hoechst fluorescent dye was used as a counterstain for IHC detection of the protein, vimentin. Fixed-tissue staining with Hoechst dye and an antibody.Formalin-fixed paraffin … constellation arktosNettetHoechst 33342 is a bisbenzimidazole dye with high specificity for binding to double-stranded DNA (preferentially binds to A-T base pairs). This dye is very useful to label double-stranded DNA and thus to visualize nuclei. Hoechst 33342 can be excited at ~355 nm by a UV light source (eg, UV laser beam or a mercury arc-lamp). constellation apts las vegasNettetHoechst var med å starte IG Farben sammen med BASF, Bayer og tre andre selskap. Dette utgikk fra det samarbeid som hadde startet i 1916 og som opprinnelig var startet … constellation audio pictor reviewNettet25. sep. 2024 · Photocaged Hoechst Enables Subnuclear Visualization and Cell Selective Staining of DNA in vivo Carina A. Lämmle, Carina A. Lämmle orcid.org/0000-0002-2072-6752 Department of Chemical Biology, Max Planck Institute for Medical Research, Jahnstr. 29, 69120 Heidelberg, Germany These authors contributed equally … ed rich electricianNettetHoechst stains are part of a family of blue fluorescent dyes used to stain DNA. These Bis-benzimides were originally developed by Hoechst AG and numbered all compounds, so the dye Hoechst 33342 is the 33342th compound produced by the company. There are 3 related Hoechst dyes: Hoechst 33258, Hoechst 33342 and Hoechst 34580. ed riche photographyNettetHoechst stains are part of a family of blue fluorescent dyes used to stain DNA. These bis-benzimides were originally developed by Hoechst AG, which numbered all their … edrich avenue oldbrookNettetThe Hoechst stains are a family of fluorescent stains for labeling DNA in fluorescence microscopy. Because these fluorescent stains label DNA, they are also commonly used … constellation automotive group leadership