WebSep 17, 2003 · Phospho-specific flow cytometric analyses correlated well with Western blotting, providing cross platform validation of the technique. Conclusions Measuring phosphorylation events by flow cytometry provides a rapid and efficient way to measure kinase cascades in individual cells. WebProtocol Outline. Prepare all reagents and samples as instructed in the manual. Add 100 µl of sample or positive control to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 µl of prepared primary antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared 1X HRP-Streptavidin to each well.
Protocol for Phospho-Flow Cytometry Preparation
WebPhospho-S6 Ribosomal Protein (Ser235/236) Antibody detects endogenous levels of ribosomal protein S6 only when phosphorylated at serine 235 and 236. This antibody does not detect ribosomal protein S6 phosphorylated at other sites. Species Reactivity: Human, Mouse, Rat, Monkey, S. cerevisiae WebProduct Details Storage & Handling This buffer solution should be stored between 2°C and 8°C. Application ICFC - Quality tested ICC Recommended Usage For cell fixation, use 250 µL fixation buffer per tube and leave it in the dark for 20 minutes at room temperature. my phone blacked out and won\u0027t turn on
Phospho definition of Phospho by Medical dictionary
WebFunction. ERK1/2 are broadly expressed protein kinases that are involved in proliferation, differentiation, motility, and cell death. In response to extracellular stimuli, ERK1 and ERK2 are phosphorylated at Thr202 and Tyr204; and in turn transduce signals to downstream substrates. Interaction. ERK1/2 phosphorylates a wide range of downstream ... WebCells were then intracellularly stained with anti-EGFR Phospho (Tyr845) recombinant (clone QA19A93) PE. ... For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each ... WebFlow cytometric analysis of Jurkat cells, untreated (blue) or treated with Hydrogen Peroxide (11mM, 1 min; green) using Phospho-SLP-76 (Ser376) (E3G9U) XP ® Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb … my phone black screen